Input Parameters

Carbohydrate Database
The user has to select the carbohydrate database from the drop down menu. There are currently four carbohydrate databases to choose from: HIV, Pituitary hormone, Serum/Plasma proteins, and all entries.

Peptide Sequence
The known peptide sequence must be entered in this box.

Peptide Mass
If the peptide sequence is not known, the mass corresponding to the singly charged species is entered. (Note that cyateine modification and variable modification are not needed in this case)

Cysteine Modification
Cysteine modification: none, carbamidomethyl,or carboxymethyl

Variable Modification
These are known Amino acid residue modification: none, carbamylation,oxidation of methionine, and deamididation of glutamine or cysteine.

Charge State
The charge state of the ions: MH⁺, MH²⁺, MH³⁺, MH^-, MH^2-, MH^3-

Mass Tolerance
The mass error of the data in ppm.

Charge Carrier
The ion adducts. For example, MH⁺ for protonated species and MNa⁺ for sodiated species.

PeakList
Experimental masses to be analyzed are entered separated by new lines or copied and pasted from an excel file.

To perform a search, paste the peaklist in the search form, specify the carbohydrate database to search, mass error, peptide sequence (or peptide mass), cysteine modification, charge state, mass tolerance, charge carrier and press the submit button. The query result reports the theoretical mass that matches the experimental mass within the specified mass error. This is displayed in a tabular format in the order, Peak List Mass, Database Mass, Mass Error, Database Entry.